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Follicular Dendritic Cell Secreted Protein (FDC-SP) Recombinant, Antibody & ELISA Kit
A small secreted protein of expressed in human tonsillar germinal centers (GC) has immunomodulatory activities on B cells.
Follicular dendritic cell secreted protein (FDC-SP), has been found to inhibit osteogenic differentiation of human periodontal ligament cells (hPDLCs) in recent studies. Based on these findings, we further investigate its effect on phenotype expression of hPDLCs in the present study, aiming to contribute to a better understanding of the biological functions governing FDC-SP-induced hPDLC differentiation. hPDLCs were firstly identified with immunocytochemical staining, followed by transfection with FDC-SP lentiviral vector. Western blot analysis was used to confirm the expression of FDC-SP. Then the influence of FDC-SP transfection on hPDLC proliferation, osteogenic and fibrogenic phenotype expression was evaluated at the mRNA and protein level. Procollagen type I c-peptide production was measured and alizarin red staining was then conducted to demonstrate effect of FDC-SP on functional differentiation. We found that hPDLCs could be successfully transfected with FDC-SP. Cell proliferation and cell cycle tests indicated that transfection with FDC-SP did not affect hPDLC proliferation. Moreover, according to real-time PCR and Western blot results, expression levels of type 1 collagen alpha 1, type 1 collagen alpha 2 and type 3 collagen were upregulated while that of osteocalcin, osteopontin, and bone sialoprotein were downregulated in FDC-SP transfected cells. In addition, hPDLCs overexpressing FDC-SP exhibited higher PIP production than the controls. Our findings demonstrate that transfection with FDC-SP has negligible adverse effect on proliferation of hPDLCs and imply the biological function of FDC-SP as a fibroblastic phenotype stabilizer by inhibiting hPDLCs differentiation into mineralized tissue-forming cells, thus regulating regeneration in periodontal tissue engineering
Xiang L, et al. J Cell Biochem. 2014 May;115(5):940-8

Effect of FDC-SP on the phenotype expression of cultured periodontal ligament

INTRODUCTION: Recently, a novel protein, follicular dendritic cell secreted
protein (FDC-SP), has been identified in human periodontal ligament (PDL) tissue
and a biomolecular study suggested that the expression of FDC-SP might be
associated with the expression of the PDL phenotype. The purpose of this study
was to test the effect of FDC-SP on the proliferation and phenotype of PDL cells.
MATERIAL AND METHODS: Periodontal ligament cells obtained following the 3(rd)
passage were exposed to various concentrations of FDC-SP. The cell proliferation
was monitored by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium
bromide(MTT) assay. Then, as a measure of osteogenic activity, the alkaline
phosphatase (ALP) activity was recorded after 4, 7, and 14 days using
p-nitrophenylphosphate as a substrate. Finally, total RNA was extracted and
RT-PCR was performed for gene analysis.
RESULTS: The results indicated that PDL cells exposed to 50 ng/ml FDC-SP could
proliferate more rapidly. RT-PCR results showed that the mRNA expression of
epidermal growth factor receptor (EGFR) was obviously upregulated and the mRNA
expression of osteocalcin (OCN) and bone sialoprotein (BSP) were downregulated in
PDL cells exposed to FDC-SP. Moreover, two groups of PDL cells exposed to FDC-SP
showed a significant decrease of ALP activity during all the culture days.
CONCLUSIONS: In sum, the findings observed in this study suggest that FDC-SP in
PDL cells could positively affect the proliferation and act as a fibroblastic
phenotype stabilizer by inhibiting their differentiation into mineralized
tissue-forming cells.

Wei N, et al. Arch Med Sci. 2011 Apr;7(2):235-41. doi: 10.5114/aoms.2011.22073. Epub 2011 May
  FDC SP (Human)ELISA Kit
Code No.: SK00319-01
Size: 480 T
Price: $2200.00 USD
Standard Range: 50-800 ng/ml
Sensitivity: 10 ng/ml
Sample Type: recombinant protein derived from E. Coli
Sample require: 100ul per well
 Data Sheet: PDF
Anti FDC SP (Human)IgG
Code No.: A00319-01-100
Size: 100 ug
Price: $460.00 USD
Host: Rabbit
Antigen:FDC SP (H) Rec.
Ab Type: Polyclonal IgG
Purification: Protein A
Applications: indirectly E, IHC,
Working Dilution: IHC 1: 500 (2 ug/ml)
Data Sheet: PDF

human FDc SP recombinant

Human FDC SP Recombinant
Code No.: 00319-01-100
Size: 100 ug
Price: $460.00 USD
Protein ID:Q8NFU4
Gene ID:260436
MW:7.5 KD
Tag: His Tag on N-Terminus
Expressed: E. Coli
Purity: 95%
Data Sheet: PDF


Code No.
Price ($)
FDC-SP (Human) ELISA Kit SK00319-01 480 T 2220.00 
FDC SP (Human) Rec.
100 ug
FDC SP (Human) Rec.
1000 ug
FDC SP (Human) Rec.. Biotinylated
50 ug
Anti Human FDC SP Rabbit IgG
100 ug
Anti Human FDC SP Rabbit IgG Biotinylated
50 ug