| Adipose Triglyceride Lipase (ATGL) ELISA | |
Alternative name:
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| Higher levels of ATGL are associated with exercise-induced enhancement of lipolysis in rat epididymal adipocytes | |
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BACKGROUND: In adipose
cells, adipose triglyceride lipase (ATGL) catalyzes the first step in adipocyte
triacylglyceride hydrolysis, thereby regulating both basal and
hormone-stimulated lipolysis. However, little is known about the
molecular mechanism(s) underlying habitual exercise-induced adaptive
modulation of ATGL in white
adipocytes via alteration in transcription regulator and lipolytic
cofactors. METHODOLOGY/PRINCIPAL RESULTS: Male Wistar rats were randomly divided into 2 groups a sedentary control group (CG) and a habitual exercise group (EG). The EG was subjected to running on a treadmill set at 5 days per week for 9 weeks. The CG was not subjected to running on a treadmill. In the EG, levels of ATGL mRNA and protein were elevated with a significant increase in lipolysis compared with the CG, accompanied by a significant increase in associations of CGI-58 with ATGL protein. Under these conditions, an upregulation of peroxisome proliferation-activated receptorg-2 (PPARg-2) was observed. In the EG, the addition of rosiglitazone further significantly increased the levels of ATGL protein compared with the CG. However, attenuated levels of the ATGL protein in adipocytes were obtained by the addition of insulin, which is known to inhibit the expression of ATGL in both types of groups. Actually, levels of plasma insulin were significantly reduced in the EG compared with the CG. CONCLUSIONS:These data suggest that elevated levels of ATGL are involved in the exercise-induced enhancement of lipolysis in primary adipocytes. The exact mechanism(s) underlying this phenomenon is associated, at least in part, with upregulated transcriptional activation of PPARg-2. In addition, exercise-induced lower circulation levels of insulin also correlate with habitual exercise-induced higher levels of ATGL in primary adipocytes. |
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| Ogasawara J et al. PLoS One.2012;7(7) | |
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Human ATGL ELISA Kit Code No.: SK00576-01 Size: 96 T Price: $420.00 USD Standard Range: 0.78-100 ng/ml Sensitivity: 200 pg/ml Sample Type: tissue homogenates, cell cultures Sample require: 100ul per well Protein ID :Q96AD5 Gene ID : 57104 Intra-CV: 4-6% Inter-CV: 8-12% Data Sheet: PDF |
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Human ATGL His Tag Rec Code No.: 00576-01-100 Size: 96 T Price: $360.00 USD Protein ID :Q96AD5 Gene ID : 57104 Tag; His Tag on N-terminus MW: 73 KD Data Sheet: PDF |
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Anti Human
ATGL Antibody Code No.: A00576-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human ATGL, Rec Ab Type: Purified IgG Purification: Protein A Applications: E, IHC, WB Working Dilution: IHC 2-4 ug/ml Data Sheet: PDF |
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Anti Human
ATGL Antibody Code No.: A00576-01-100 Size: 100 ug Price: $220.00 USD Host: Rabbit Antigen: human ATGL, Rec Ab Type: Purified IgG Purification: Protein A Applications: E, IHC, WB Working Dilution: IHC 2-4 ug/ml Data Sheet: PDF |
| Anti Human
ATGL Monoclonal Antibody Code No.: A00576-06-100 Size: 100 ug Price: $260.00 USD Host: Mouse Antigen: human ATGL rec Ab Type: Purified IgG Purification: Protein G Applications: E Working Dilution: 0.2 ug/ml) Data Sheet: PDF |
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| Name | Code No. |
Size |
Price ($) |
| ATGL (Human)ELISA Kit | 96 T |
420.00 |
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| ATGL (Human)Recombinant | 10 ug |
80.00 |
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| ATGL (Human)Recombinant | 100 ug |
360.00 |
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| ATGL (Human) Recombinant | 100 ug |
360.00 |
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| Anti Human ATGL IgG | 100 ug |
220.00 |
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| Anti ATGL (Human) Monoclonal Antibody | A00576-06-100 |
100 ug |
260.00 |